Sentence examples for binding and the ligation from inspiring English sources

Recombinant XRCC4 protein stimulates DNA binding and the ligation activity of DNA ligase IV in vitro (Grawunder et al, 1997).

The method has advantages relative to both immunoPCR (because no signal is produced by background binding) and the proximity ligation assay (PLA) (because only one epitope, rather than two epitopes, on a protein surface must be bound).

The presence of the -256A>TG indel disrupts the binding and ligation of the FCGR3B#1 MLPA probe.

For example, the use of secreted frizzled-related proteins (SFRPs) that antagonize Wnt signaling by competing for Wnt binding and preventing ligation to the frizzled receptor reduces infarct size and improves cardiac function.

The molecular mechanism involved in clarithromycin resistance consists of mutations in the sequence of the H. pylori domain V of the 23S rRNA fraction which is involved in the peptiltransferase ribosome binding site preventing the ligation of the macrolide to the rRNA [ 30].

Other key advances include the use of proximity ligation to confirm vicinity binding and the use of quantum dots, which have proven potential for cellular labelling.

In the work presented, we have designed a binding assay and used mutational analysis to investigate the contribution of each of these structural changes to binding and ligation.

We find that the loop I V kissing interaction is necessary but not sufficient for binding and ligation.

HECT ubiquitin ligases contain an N-terminal membrane-binding C2 domain, WW domains that interact with PPXY motifs in binding partners and a C-terminal HECT domain that has ubiquitin binding and ligation properties [ 15].

This ZNF domain is believed to facilitate binding and ligation of DNA breaks located near DNA secondary structures (Cotner-Gohara et al, 2008; Taylor et al, 2000) and thus may be important for targeting TDP1 to closely spaced Top1-SSBs.

We have not observed any problems with non-specific binding of proteins under the ligation conditions used here.