The fact that we observed significant changes in protein expression accompanied by less dramatic alterations in mRNA levels and comparable polymerase II binding suggested the involvement of microRNAs (miRNAs).
The cofactor-dependent Ubx peaks are associated with enrichment of Hox, Exd and Hth binding motifs indicating the involvement of the binding of several proteins.
These results suggest that Arg at position 73 is involved in LTB4 binding, while the involvement of Arg at position 53 seems enhanced in the frame of the double mutant configuration (R53T/R73A).
Besides our observations, extensive investigation of the binding of the original benzodiazepine ligand Ro5-4864 has led to the conclusion that the C-terminal region of TSPO, in particular residues 144 156 of helix V, which include both the CRAC site and the sequence preceding, plays a role in its binding, although the involvement of loop 1 is also observed.
Additional terms corresponded to nucleotide binding, suggesting the involvement of transcription factors (including WRKY, for which we found also terms in module B).
C2H2 zinc fingers (ZF) display a wide range of functions, from DNA or RNA binding to the involvement in protein-protein interactions.
We then gained an understanding of the roles played by the occupancy of the two alternate binding sites (NMBD and TMBS) by copper and the involvement of the NMBD and A domain in protein conformational transitions within the ATPase cycle.
The inhibitory activity of conjugate 30 on HGF/MET binding was moderate (IC50 ∼ 15 μM) but not unexpected given the complexity of the HGF/MET interaction and the involvement of an extensive binding interface.
Coadministration of the anti-androgen Casodex markedly decreased the Mb-induced DNA-binding complex indicating the involvement of the AR (lane5).
To confirm the interaction of MT2 with TrkA residues involved in NGF binding and to rule out the involvement of other acceptors, for example, other protein kinase receptors, in the response to MT2, similar studies were repeated on the wild type (WT), F327A, and T352A TrkA-NIH-3T3 stable transfectants.
