These data, by defining a critical role for Asp-280 in substrate binding and subunit interactions, give insights into the mechanisms of regulation of iNOS activity.
Kinetics and subunit interactions of ribulose bisphosphate carboxylase-oxygenase from the Cyanobacterium, Synechococcus sp. J. Biol.
These studies set the stage for determination of nucleotide binding and how it effects subunit interactions and quaternary structure in a class Ib RNR.
Galpha subunits bound to guanine diphosphate (Galpha-GDP) inhibit signal transduction through Gbetagamma subunits, suggesting a common interface on Gbetagamma subunits for Galpha binding and effector interaction.
Gα subunits bound to guanine diphosphate (Gα-GDP) inhibit signal transduction through Gβγ subunits, suggesting a common interface on Gβγ subunits for Gα binding and effector interaction.
Here, we report 15N-nuclear magnetic resonance heteronuclear relaxation analysis to characterize the movements of single-stranded (ss) DNA binding and protein interaction modules in the RPA70 subunit.
This indicates that, in addition to dimerization, proper subunit interaction is required for substrate binding.
Each sub-family was found to have one or more central core regions with extensive homologous motifs (Addtional file 3: Figure S3), that have been reported to be important in subunit interactions and DNA binding in yeast and mammals [ 29, 48, 70, 78].
The NF-YC sub-group contained a central domain of approximately 80 AAs that was highly conserved across the different members, and which has been shown to be important for both DNA binding and interactions between NF-Y subunits (Additional file 3: Figure S3) [ 64].
Oxygen binding encompasses structural changes at the interfaces between the α1β2 and α2β1 dimers, but also subunit interactions are important for the oxygen binding affinity and stability.
