In order to guide experimental design, a kinetic model was built to explore how the affinity and valency of dual-ligand liposomes affect the binding and selectivity of delivery to cells with various receptor expression.
The effects of different basic moieties with regard to binding and selectivity towards the five distinct muscarinic receptor subtypes were investigated.
We also present the structure of TbHpHbR in complex with HpHb, revealing the molecular basis for ligand binding and selectivity.
After labeling with reporter fluorescent groups, the oligonucleotides preserved their abilities to fold into a quadruplex structure and to interact with metal cations with binding affinity and selectivity comparable with that of unmodified oligonucleotides [ 29, 40, 172, 173].
Application of our scFv homodimer conjugate reveals that CEA binding activity and selectivity is maintained after conjugation, with a shift in fluorescence being observed only on the A375CEA cell line.
The most recently described tau ligand came from the Siemens Molecular Imaging Groupp (recently acquired by Avid/Lily) screening over 900 compounds to determine which had both higher binding affinity and selectivity for tau tangles compared with Aβ plaques [ 103].
G-Quadruplex DNA binding affinity and selectivity studies by FRET-melting assays showed that NSC748392 and NSC478393, with aliphatic amine side chains, are good G-quadruplex ligands but not selective, whereas a C-11 aromatic side chain, as in NSC748394, increases selectivity although with decreasing binding affinity.
