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Our analyses revealed that the chimeras could be separated into four different groups in terms of HDL binding and selective uptake of HDL-CE.
In the final group, SC 92 98), SC 219 223), and SC 282 287) displayed extremely low levels of HDL binding and selective uptake of HDL-CE despite their high levels of cell surface expression (41 59% higher than that of wild-type SR-BI).
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Further, our data support the notion that while a specific fold or conformation of the C-terminal half of the EC domain of SR-BI is required for mediating proper binding to HDL and selective uptake of HDL-CE, disruption of this region does not significantly impact the ability of this receptor to facilitate the release of free cholesterol from cells to HDL.
Plasma was monitored after the initial injection for 24hours, after which mice were euthanized and selective uptake measured in tissues.
In first trimester trophoblasts, both HDL degradation and selective uptake from HDL are high.
To further define and characterize extracellular subdomains within SR-BI that are required for cholesterol transport, we constructed a series of 16 SR-BI/CD36 chimeric receptors that span the entire EC domain of SR-BI to delineate regions that are essential for HDL binding and HDL-CE selective uptake.
SR-BI mediates the high-affinity binding of HDL particles and the selective uptake of HDL derived lipids into cells [2].
Simplistic metabolic pathways, the cybernetic model, and the selective uptake model have been presented.
Here, by its bridging function, EL facilitates HDL particle binding and uptake [5,6], as well as selective uptake of HDL cholesteryl esters [6].
In order to begin to develop a more complete understanding of the structural factors in the carbohydrate domain that conduce to selective, potent binding and uptake by tumor cells, a focused library of structural analogues of the natural bleomycin disaccharide has been synthesized.
A receptor for the selective uptake and degradation of proteins by lysosomes.
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