Also, it can be utilized for further binding analysis and screening of inhibitors of hPRR.
This specificity also allows for binding of parasite glycans and screening of an array of bacterial outer membrane oligosaccharides confirms that the receptor binds to a subset of these structures with appropriately exposed GalNAc residues.
In addition to the primary binding site, screening of glycan arrays and structural analysis of C-type CRDs in complex with glycan ligands has increasingly demonstrated the importance of contacts outside of the primary sugar-binding site in extended or secondary binding sites [ 14].
The difference in these two dielectric environments could affect the exciton binding energy through screening of the Coulomb interaction between electrons and holes.
Using this non-radioactive tracer, we established a high throughput cell-based receptor-binding assay for screening of novel RXFP2 agonists or antagonists in future studies.
Hybridoma supernatants were collected and screened for antigen binding and neutralization of VEGF/VEGFR-2 interaction.
An alternative technology that has received attention from several laboratories, including our own, is to employ simple protein binding screens and libraries of bead-displayed compounds.
To identify the contribution of the diverse Mx2 domains to capsid binding, we generated and screened a series of deletion constructs of Mx2 with an N-terminal His-Sumo-tag (Fig. S1A) and characterized the expression, solubility, stability, and oligomerization behavior of these constructs (Table S1).
As expected, TRP601 had no substantial activity outside the caspase family, as found in a binding screen of 110 different receptors, transporters and ion channels, and 56 enzyme activity assays, including calpains, various cathepsins (B, H, G, L), granzyme B, and several glutamatergic sites such as N-methyl--aspartatic acid and α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (Table 1).
In contrast to our hypothesis-free TFBS screen, the discovery of mtDNA binding and determination of the subcellular localization of these additional TFs were motivated by prior interest in the function of these factors.
