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Moreover, the most enriched GO terms associated with the consistent SDE mRNAs for molecular function were binding and protein binding (Fig. 1D).
In contrast, genes related to basic cellular processes ("nucleus", "DNA binding" and "protein binding") are all significantly under-represented in CNV-region genes.
The most common were DNA/RNA binding and protein binding.
In cellular function, DNA binding and protein binding were the most common terms with 18%, and 17% respectively.
While MCF-Exo contains higher nucleic acid binding and protein binding and protein transport activity, the MDA-Exo contains proteins with more catalytic activity.
Well documented functions for non-coding RNA include regulation of the genome (through chromatin modification, DNA binding, and protein binding) and of cellular differentiation during development [ 39– 41].
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In the molecular function category, the enriched GO terms included binding, nucleic acid binding, and protein-binding.
RNA-binding and protein-binding functions were also over-represented in active PAC genes.
Not surprisingly, de Lima et al. also reported more missense mutations in the DNA-binding and protein-binding domains, encoded by exons 3 4 and 7 9, respectively.
Whereas the missense mutations underlying VWS were almost evenly divided between the DNA-binding and protein-binding domains, the vast majority of the missense mutations found to be associated with PPS arose in the DNA-binding domain.
In addition, some of the predicted targets were annotated as being involved in various classes of molecular functions, including binding proteins (DNA-binding, RNA-binding, ATP-binding and protein-binding), calcium ion transport proteins, and enzymes (protein kinase, synthetase/ligase, acetate-CoA ligase, glycoside hydrolase and oxidoreductase).
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