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This so far unobserved subsite distortion is incompatible with productive substrate binding and processing.
All-trans- and 11-cis-retinol derivatives substituted with various electron-withdrawing groups at C12 were designed to be affinity labels for retinol binding and processing proteins.
Indeed, nucleic acid binding and processing enzyme genes represented the largest functional category with several helicases among them.
This study clearly shows the dynamic structures within the RNA-binding domains, which provides additional insights on mechanism of asymmetric RNA binding and processing.
24% of genes are potentially involved in direct gene expression regulation, DNA/RNA binding and processing.
We explored more radical mutants S42Q and A52D, which immediately flank the core peptide; both mutants significantly disrupted binding and processing.
Similar(30)
We further mapped mTOR-regulated phosphorylation sites in eukaryotic translation initiation factors and identified that phosphorylation of Ser214 on eIF5B enhanced the binding of eIF5B to the RNA-binding and processing proteins including Nat10.
The weakness of all these models is that they presuppose secondary B cells binding to and processing secondary antigens in the absence of T cell help.
The MRE11 and RAD50 proteins form a heterotetramer which contains two DNA-binding and processing domains that can bridge free DNA ends [ 8].
However, the display levels of two of the tested proteins, GFP and 2224, fused to the 202 08 intein did exceed that of the respective unfused proteins, and the 2224 scFv had an improved EGFR-specific binding capacity, indicating beneficial folding and processing effects of the intein fusion partner.
The whole detection of the real binding motifs and processing of the full size data of several megabytes finished in a few minutes.
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