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We then mutated the seed region in MEF2C for miR-21 binding and performed the luciferase assays as above.
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It gives a great opportunity to monitor binding and perform loading of the amino compounds onto the reactive surface.
For example, for predicting the transport and binding proteins functional class, we labeled all the other 12 functional classes as 'not transport and binding proteins' and performed a binary classification of transport and binding proteins against 'not transport and binding proteins'.
Moreover, to demonstrate ProBiS' unique ability to detect and align similar binding sites in the absence of global fold similarity, the authors examined 10 pairs of protein structures, where the two members of each pair exhibited different folds but had known similar binding sites and performed a similar function.
For Ubx, we used the top 300 binding peaks and performed motif discovery analysis using nestedMica [50] for the embryo and haltere data separately.
TS was involved in DNA binding studies and performed cloning and Western blotting studies.
We mutated the candidate transcription factor binding sites and performed the luciferase assay again.
We took the average log2 value of the read counts within each detected binding region and performed quantile-normalization.
We also simulated in vivo conditions immobilizing ephrinA1-Fc on high binding plates and performing adhesion with PC3 cells.
The proteins described to regulate Drosha and/or Dicer processing by interacting with the TL of specific miRNA precursors possess known RNA-binding domains and perform additional functions in RNA metabolism.
CTLDs in the members of these groups have lost their carbohydrate-binding activities, and perform functions that have, apparently, evolved after evolutionary separation of tetrapods from fish, or which are mediated by other proteins in fish.
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