We did not try to define genes that are bound or unbound by the MSL-complex, but rather select two extreme groups (one with very strong binding and one with very weak or no binding).
Fig. 2G shows an example for two solutions of ϕ, one with KS=0.1 (blue), reflecting a gene with low levels of Sox2 binding, and one with KS=10 (purple), reflecting a gene with high levels of Sox2 binding.
Rules 2 and 3 overlap with the second group of membrane and DAG-binding residues indicating that two different kinds of function conservation appear here: one associated with membrane binding and one associated with other activity.
Therefore, in terms of our model we considered them as two distinct binding sites within ER - one for ligand binding, and one for binding with other monomers.
Enrichment analysis revealed overrepresented biological process terms associated with skeletal muscle regeneration, cell differentiation, chemotaxis and the bacteria-induced defense response, molecular function terms associated with chemokine activity and heparin binding, and one cellular component term associated with the myosin complex, as first identified at 14 days PI.
Most photoswitches are based on constraints that make one state closed or incompatible with binding and one state binding-competent.
We have also applied FEATURE to function prediction in RNA structures with two magnesium binding models, one for diffuse binding and one for site-specific binding [ 30].
After washing three times with 10 mL of binding buffer and one time with buffer containing 100 mM imidazole, 6His-ZapA was eluted in 5 times 5 mL elution buffer (20 mM phosphate, pH 7.4, 150 mM NaCl, 500 mM imidazole, 10% glycerol).
Six specimens with binding bars and one specimen without binding bars were tested to examine the effects of horizontal spacings and diameters of binding bars, and width-to-thickness ratios of steel plates on the failure modes, maximum strength, and ductility of L-shaped CFT stub columns.
Although the exact reason for this is unclear, it may be that there are two populations of binding sites, one with high affinity and a second with lower affinity and greater recognition sequence degeneracy that is therefore more abundant in the genome.
Mr. Reyes continued his siege of women in their 20's, following them into their homes, tormenting and assaulting, stabbing and beating them, binding more than one with the same knot that he apparently fashioned with the jogger's T-shirt to tie her up in the muddy ravine where she was found.
