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Most of the binding functions were at the intracellular level rather than external and included nucleotide and nucleic acid binding, protein binding and ion binding.
Nucleic acid binding, protein binding, and ion binding functions were predominated in the binding category.
In terms of molecular function, transferase activity, nucleotide binding and ion binding related sequences were the top three GO terms in the Sanger-EST assembly.
They were represented by a number of GO terms involved in binding, with 1,777 and 1,430 ESTs annotated as "nucleic acid binding" and "ion binding" respectively.
The 18 most abundant GO terms into which E4/E0 and E24/E0 unigene sets were distributed included many abiotic stress-related categories, such as "transferase activity", "nucleic acid binding", "nucleotide binding", and "ion binding".
Similar(55)
The ontology of the non-expressed rye genes that were found by a BLASTx search in the GenBank NR database was varied, but the majority of these code for proteins with catalytic activity and proteins with nucleotide-binding and ion-binding activity (Additional file 3: Table S3).
Only 4 significant GO molecular functions were observed, and they were involved in general binding activities (binding, protein and ion binding, catalytic activity, FDR & 0.05).
The downregulated transcripts were mainly grouped into hydrolase, oxidoreductase and binding, especially nucleic acid, protein and ion binding.
The GO terms that were most significantly enriched were "metal and ion binding" and "DNA binding", the latter including genes encoding for proteins with roles in transcription and regulation of transcription.
Its GO annotated cellular location is component integral to membrane and the GO category of molecular functions lists ATP binding, calcium ion binding and transmembrane receptor protein tyrosine kinase activity.
Chitin binding, calcium ion binding and hydrolase activity were the most significantly over-represented attributes and nucleic acid binding was identified as the most significant under represented attribute.
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