Sentence examples for binding and internalization with from inspiring English sources

We performed C. jejuni binding and internalization with the 3T3 MEF WT and 3T3 MEF KO cells (Additional file 10: Figure S10).

To determine if the presence of caveolae might potentiate the number of C. jejuni internalized, binding and internalization assays were performed with Caco-2 cells that were transfected with a plasmid expressing caveolin-1 (Additional file 9: Figure S9).

Wnt stimulation induces the casein kinase 2 (CK2 -dependent phosphorylation of LRP6 at S1579, promoting its binding to Dab2 and internalization with CK2 -dependent

To avoid binding and internalization of FBS with HGNs, a FBS-free medium was used.

Cells were incubated for 20 minutes with bead suspension at a final concentration of 2×108/ml with inhibitors or controls for binding and internalization, washed three times with HBSS/0.3% BSA, and incubated an additional 20 minutes in assay buffer with inhibitors or controls for further internalization.

C. jejuni binding and internalization assays were performed with HeLa, Caco-2, and 3T3 MEF cells as outlined previously [ 60].

For binding and internalization, cells were preincubated with Rh-EGF at 4°C to allow for binding, followed by removal of unbound Rh-EGF incubated for the indicated times and concentrations with Rh-labeled EGF at 37°C, and then washed with either PBS or an acidified buffer as described previously [ 30], followed by ALDH1 staining.

Nonspecific binding and internalization was determined by coincubation with an excess of 6.7 nM unlabeled MG1.

Phage display biopanning in Round 1 is performed using established protocols with cell binding and internalization as the selection criteria.

The peptide has been radiolabelled with I-125 and radioligand binding and internalization assays have been carried out in cells that overexpress αvβ6 and compared with that of the 111In-DTPA-A20FMDV2 studied previously.

Binding of Aβ peptides to the plasma membrane was sufficient to induce trafficking inhibition where peptides with reduced plasma membrane binding and internalization had lesser effect on mitochondrial motility.