To avoid binding and internalization of FBS with HGNs, a FBS-free medium was used.
As expected, the cellular binding and internalization of all radiometalated PSMA ligands in this study increased over the time of observation (1 h).
Internalization experiments on LNCaP and PC-3 cells indicated specific cell surface binding and internalization of [68Ga]6 and [68Ga]7 (Figure 2a).
The kinetics of membrane binding and internalization of [125I]SGMIB-MNT by A431 and D247 MG cells was investigated at selected time points up to 24 h.
Epithelial cell adhesion molecular (EpCAM) specific antibody fragment appending enhanced binding and internalization of Onconase toward EpCAM positive cancer cell and increased its tumor accumulation and retention.
Some of these may be due to mannose receptor, or potentially also FcγR-mediated binding and internalization of ADCs, which could result in "off-target" toxicity issues (Gorovits and Krinos-Kiorotti, 2013; Beck et al., 2017).
The specific binding and internalization of the recombinant scFv apoptin into the target cells through the scFv, is essential for effective delivery of apoptin and its potential therapeutic function.
The critical role of heparan sulfate proteoglycans (HSPGs) in mediating cell binding and internalization of polymer-coated AdV was also demonstrated by evaluating transduction in HSPG-defective recombinant CHO cells.
Specific binding and internalization of labelled conjugates was evaluated in CD44v6-expressing SCC cells in vitro, and in vivo binding specificity and biodistribution studies were then performed using 111In- or 125I-labelled Fab fragments in a dual-isotope study in tumour-bearing mice with xenografts of varying CD44v6 expression.
