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Upon binding and fixation of purified viral particles to Hela cells, an immunostaining protocol was performed to confirm that viral particles incorporated the scFvFc fusion proteins.
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With the possible exception of this site, the observed weak HSF binding in the RT samples may reflect transient HSF binding, HSF binding in a subset of cells, and/or is the result of the induction of a mild HS response brought on by the initial harvesting and fixation of the cells.
TMI and BMvR conducted the sampling and fixation of specimens.
TMI conducted the sampling and fixation of specimens.
This hampers an optimal insertion and fixation of the cup.
(ii) This incorporation provides a very clear demonstration of the essential mechanistic role for obligatory, reversible loss of H2 upon N2 binding and thus of the eight-electron stoichiometry for nitrogen fixation by nitrogenase embodied in eq 1.
Still, in the case of Apaf-1, there is a clear pattern of emergence and evolutionary fixation of several Asp-Asp motifs (Fig. 10) that, as the modeling suggests, might be involved in binding the lysine residues of cytochrome c.
Fixation of a mutant implies first fixation within the group and then fixation of the group's strategy in the population.
Such a negative relationship can be explained if we assume that binding site turnover is due to accidental fixation of slightly deleterious mutations and the selection of compensatory mutations.
Similarly, mannose-binding protein deficiency in humans shows that antibody-independent fixation of complement triggered by binding of serum mannose-binding protein to sugars on the surface of pathogens is an essential part of the innate immune response in young infants [ 5].
This model predicts that, in contrast to prevailing beliefs, even small selective benefits per locus can drive concurrent fixation of transcription factor and binding site mutants under a broad range of conditions.
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