Other amino acid exchanges in the TPI protein, such as Cys41Tyr and Ile170Val, have been predicted to interfere with both the substrate binding and the dimerization site possibly affecting the catalytic activity plus molecular stability of TPI; both pathogenic TPI variants have been identified in unrelated European kindreds [2], [13], [15].
The drug-induced protease mutations perturb the mechanistically crucial hinge axes that involve key sites for substrate binding and dimerization and mainly coordinate the intrinsic dynamics.
In both birds and mammals these amino acid residues include known structural and functional sites, involved in ligand binding and dimerization.
Sites b5, b9, b12, b13, H110, and H205 are largely invariant among network members due to site-specific restrictions in E-box DNA binding and dimerization stability.
Mechanistically, most of them act through blockage of phosphotyrosine residue binding sites called Src-homology 2 (SH2) domains necessary for STAT3 receptor binding and dimerization.
The inserted sub-domain is shown to be involved in substrate binding and dimerization.
The N-terminal region of the receptors is involved in membrane localization, ethylene binding, and dimerization.
It has been proposed that heparin binding site and dimerization of serglycin core together with glycosaminoglycan chains regulate the binding of serglycin with other molecules [ 23, 29].
This derivative retains LR1, nuclear localization sequence (NLS), and the DNA-binding and dimerization domains.
NF-κB subunits are sensitive to small variations in the κB sites because the RHR region of NF-κB, which is responsible for both DNA binding and protein dimerization, is bimodular (including the N-terminal domain and C-terminal dimerization domain) and connected through a small linker peptide.
