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To map the domains of Sdo1p responsible for the binding and dimerization of the 60S subunit, we further characterized the binding of individual domains of Sdo1p to the 60S subunit.
It has been shown in bacteria that DNA binding and dimerization of the Fe/S-containing FNR protein from Escherichia coli are regulated by oxidation [44].
15, 23 It stimulates proliferation of endothelial cells, through binding and dimerization of cell surface receptors.
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Moreover, the YjeF_N domain was required for RNA-binding and dimerization of Edc3, whereas it also influenced regulation of Edc3 target mRNAs (Ling et al. 2008).
It has been proposed that heparin binding site and dimerization of serglycin core together with glycosaminoglycan chains regulate the binding of serglycin with other molecules [ 23, 29].
The I domain is relatively less conserved, and contributes to the DNA binding specificity and dimerization of these proteins [ 12].
In particular, comparison of the DNA binding and dimerization domains of SlmA with QacR yielded a root mean squared deviation (RMSD) of 1.9 Å and 3.9 Å, respectively.
The constitutively active mutant construct FLAG-C2/ATF2 [ 37] that contains a FLAG tag at the N-terminus, followed by the constitutively active activation domain of CREB2 and the DNA binding and dimerization domains of ATF2 was generously provided by G. Thiel (University of the Saarland Medical Center, Homburg, Germany).
Coupled with our observation that TraR proteins from the two major clades cannot efficiently cross-dimerize, our results suggest that both DNA binding and dimerization domains of TraR proteins are structurally and functionally conserved within, but not between the clades.
(c) The DNA binding and dimerization domains of BES1 (1–89) and RD26 (1 140) interacts with each other.
Although different at their 3' untranslated regions, both cDNAs predict a protein with primary amino acid sequence homology to the previously described amphipathic helix-loop-helix DNA binding and dimerization motif of the Ly1-1, MyoD MyoD, immunoglobulin enhancer binding, daughterless, and achaete-scute families of genes.
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