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The hydrophobic nature of Mycobacterium tuberculosis cell wall prevents binding and diffusion of drugs [ 20].
Moreover, mycobacterium has a lipid rich cell wall, which prevents binding and diffusion of most of the drugs [ 8].
In this study we explored cholesterol-mediated binding and diffusion of DNA origami structures on supported lipid bilayer membranes and utilized it to create hierarchical assemblies and arrays of such structures with sizes of several micrometers.
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Baylor, S. M. & Hollingworth, S. Model of sarcomeric Ca2+ movements, including ATP Ca2+ binding and diffusion, during activation of frog skeletal muscle.
Figure S2: Ca2+ dependence of lipid binding and diffusion.
Single molecule tracking of Sox2 binding and diffusion dynamics in EnCs vs HCs indicates that in contrast to the previously estimated fraction (∼74%) of Sox2 molecules engaged, on average, in 3D diffusion, the majority (∼64%) of Sox2 molecules within EnCs were found to be in a chromatin bound state.
Moreover, the findings illustrate the power of single-molecule binding and diffusion studies, in concert with activity studies, to reveal the hidden steps in the activation mechanisms of membrane-bound signaling proteins.
These may include favoring high-affinity cooperative TF binding and lateral diffusion of TF binding along regulatory regions, or simply increasing of the local TF concentration in the vicinity of these regions and thereby increase the probability of functional interactions [ 38– 40].
To test the predictions of the working model, we have employed single-molecule methods to analyze the membrane binding and surface diffusion of full-length PKCα and truncation constructs lacking one or more domains.
To further investigate this mechanism, this study has utilized single-molecule total internal reflection fluorescence microscopy (TIRFM) to quantitate the binding and lateral diffusion of full-length PKCα and fragments missing specific domain(s) on supported lipid bilayers.
(C ) Whole-cell Sox2 binding and diffusion dynamics.
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