The expansion of motif-binding domains linking compact and degenerate peptides to important functions greatly increased the information processing potential of the cell by simplifying access to regulatory pathways and cell state information.
An alternative approach for coupling ligand-binding to changes in cell state is to redirect native receptor sensing and signaling into orthogonal pathways.
An important subset of the regulatory machinery is the conditionally, temporally or spatially restricted motif-binding molecules that transmit cell state information to the motif-containing biomolecule (Fig. 1c and d).
The binding properties were analyzed in competitive binding, internalization, and cell surface retention experiments.
Most photoswitches are based on constraints that make one state closed or incompatible with binding and one state binding-competent.
APC2 is involved in a series of molecular signals initiated by the binding of Wnt protein to a frizzled family receptor on the surface of the target cell and ending with a change in cell state [24], [25].
Sex-determining region Y (SRY -box binding protein-2 (SRY -box a transcription factor essential to the maintenance of the pluripotent stem cell state in embinding stem cells (ESCs) and induced pluripotent stem cells [ 1- 3].
In addition to establishing the differential binding landscape of RFs with respect to UC DMRs, we highlight the connection between RF binding and chromatin states in both a cell-type specific and independent context.
The conserved Musashi (Msi) family of RNA binding proteins are expressed in stem/progenitor and cancer cells, but generally absent from differentiated cells, consistent with a role in cell state regulation.
Such stimuli can, for example, consist of the binding of a hormone to cellular surface receptors and may initiate a series of reactions inside the cell, eventually leading to a change of cell state, directed movement, its proliferation or death.
