Sentence examples for binding and cell pellet from inspiring English sources

Compound characterization, DLS fitting, 7 T relaxivity, toxicity profiles, IC50 values, time-dependent labeling, nonspecific binding, and cell pellet T2 analysis.

BAL samples were subjected to centrifugation and cell pellet resuspended in 1 ml PBS.

Briefly, cells were harvested using 0.1% trypsin-EDTA and cell pellets were resuspended in ice-cold 1X binding buffer.

Briefly, cells were washed and harvested in PBS and cell pellets frozen in liquid nitrogen.

At days 14 18, surviving cells were washed with PBS, collected, and cell pellets were snap-frozen.

The cells were then trypsinized, pelleted at 200×g for 4 minutes and cell pellets were frozen at −80°C.

For the isolation of EhCP-A5-HA-binding proteins, the cell pellet from 1.0 × 10 EhCP-A5-HA, HA-Vps35 or wild-type HM-1-expressing cells was lysed with 1 ml of lysis buffer [50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% Triton X-100, 0.5 mg ml−1 E-64, complete mini EDTA-free protease inhibitor cocktail (Roche Applied Science, Penzberg, Germany)].

Cells were centrifuged and the cell pellet was suspended with binding buffer (100 μL) and then incubated with Annexin V-FITC (2 μL) and PI (2 μL) for 15 minutes, at room temperature in the dark.

The supernatant was decanted and the cell pellet was resuspended in 20 mL of ice-cold 1X binding buffer.

The cells were then centrifuged and the cell pellet was suspended at the concentration of 10cell per ml with binding buffer (100  μl).

Milk was centrifuged at 400g and the cell pellet removed.