These observations are in good agreement with the experimental results and can explain the binding phenomena between scorpion toxins and K+ channels at the level of molecular structure.
The simulation results are in good agreement with the previous molecular biological experiments and can explain the binding phenomena between P05 and SK channels at the level of molecular structure.
One of the three N-Hcy-lysine residues in Fbg from homocystinuric patients, N-Hcy-Lys562, is located in an unstructured region (α392-610) of the α-chain known to be involved in tPA and plasminogen binding, which can explain the abnormal characteristics of fibrin clots formed from N-Hcy-Fbg (Jakubowski 2013; Sikora et al. 2014).
Although they have similar functional EC50 values (36 and 26 nM), nothing in the functional or binding data can explain their lower potency compared to that of 1. Further, compounds 5a, 5b, and 5g have virtually identical ED50 values in the mouse HTR, yet their functional EC50s at the rat 5-HT2A receptor are 21, 34, and 80 nM, respectively.
This binding site can explain why MTs are stabilized by DCX.
In contrast, the presence of TF binding sites can explain a modest fraction of the conservation of enhancer activity.
The size of the binding cavity can explain the majority of complicating factors of CYP-mediated metabolism, which include broad specificity of individual subtypes, 1359) atypical allosteric kinetics, activation, substrate inhibition, 1362) stereoselectivity,(1362) regioselectivity, cooperativity, and formation of multiple metabolites of some chemicals.
Along these lines it is plausible that when Ire1 is monomeric, the -boxes of individual Ire1 molecules may not sustain RNA binding, which can explain the lack of mRNA recruitment under non-ER-stress conditions (Aragón et al., 2009; Figure 3D).
In addition, neofunctionalization and subfunctionalization of transcription factor binding sites (TFBSs) can explain the novelty that occurs in the regulatory region of duplicated new genes [ 10- 12].
This variation seems much greater than variability in protein binding can explain but further studies are required to determine why the clinical samples show this discrepancy.
