Sentence examples for binding and activation domains from inspiring English sources
A region of low homology of about 80 amino acids, termed the middle homology region, is found among many zinc cluster proteins and is located between the DNA binding and activation domains and may be involved in controlling the transcriptional activity of zinc cluster proteins (Schjerling and Holmberg 1996).
zFurthermore, an interaction of two potential binding partners in the used yeast system leads to the association of the DNA binding and activation domains thus forming a chimeric, functional Gal4 transcription factor.
This is a member of the nuclear hormone receptor super-family comprising classic DNA-binding, hormone binding and activation domains.
The products were cloned and sequenced and inserted in frame in the assay vectors pGBKT7 and pGADT7, carrying the binding and activation domains (Matchmaker GAL4 Two-Hybrid System 3, Clontech, Mountain View, CA).
To test this possibility, we first carried out yeast two-hybrid experiments, with binding and activation domain fusions of both FBL17 and all seven KRPs being tested.
Since such an inhibitory mechanism is not able to separate the DNA-binding and activation domains present in a single ARF, we hypothesize that a strong selection pressure on domain I of Aux/IAAs for the efficient recruitment of transcriptional co-repressors could have been a feature of Aux/IAA evolution after the appearance of canonical Q-rich ARFs.
This fact was illustrated by exchanging DNA binding domains and activation domains from one transcription factor to the next while retaining its function.
The proteins to be tested are individually fused to the DNA-binding (bait) and activation domains (prey) of the Gal4 transcription factor.
We mated each haploid yeast culture containing a single DNA-binding domain fusion to generate diploid yeast cells that express both the DNA-binding and activation domain fusions.
We amplified both the DNA-binding and activation domain inserts by PCR and sequenced the resulting products using dye-primer chemistry on capillary instruments.
This is possibly due to rearrangement and deletion of the DNA-binding domain plasmids, recombinational events between the DNA-binding and activation domain plasmids, and genomic rearrangement of the host strain.
