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SPR experiments were performed using the Biacore T100 system (GE Healthcare) and the binding analyses were carried out at room temperature in 1× Phosphate Buffered Saline (PBS) with 0.05% Tween 20.
Binding analyses were carried out in HBS-EP buffer (10 mM HEPES [pH = 7.4], 150 mM NaCl, 3.4 mM EDTA, 0.005% surfactant P20) containing 8% DMSO at a flow rate of 20 µl/min.
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FRET-based DNA binding and annealing analyses were carried out essentially as previously described (Grimme et al, 2010).
Following analyses were carried out using these 32,367 NEUROD2 binding sites (Additional file 3).
Comparative genomics analyses were carried out using xFITOM, a program for binding site search in genomic sequences [ 59, 90].
Analyses were carried out under nitrogen atmosphere.
Analyses were carried out in triplicate.
All analyses were carried out in triplicates.
Analyses were carried out at 35°C.
Statistical analyses were carried out by ANOVA.
Analyses were carried out using IBM SPSS Statistics 21.
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