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This binding affinity was trailed closely by AF750, whereas Cy3.5 and Cy5.5 both exhibited approximately four-fold less binding than GH680.
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The apparent binding affinity was derived from 3 or more experiments.
(h) Granulosa cells were harvested for ChIP analysis and the FoxO3a binding affinity was measured by real-time PCR assay.
HER2 binding affinity was determined in saturation radioligand binding assays using SKBR-3 cells (1.3 × 106 HER2/cell).
After incubation at 37 degrees C for 4 days, the binding affinity was maintained at 60% of initial levels.
Interestingly, we found that target-binding affinity was modulated by residues in the guide region.
Region-specific binding affinities were analyzed using Student's unpaired t-test.
Linear correlations between the predicted binding scores and the experimentally measured binding affinities were observed.
The process of generating antibodies with increased binding affinities is called affinity maturation.
Thus native binding affinities are measured.
BtR175-binding affinities were assessed by Biacore.
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