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Although the ligand's binding affinity was anticipated to improve when the H-bond basicity is increased (due to stronger H-bonding with the protein), we herein present data that unexpectedly revealed an opposite trend.
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The apparent binding affinity was derived from 3 or more experiments.
(h) Granulosa cells were harvested for ChIP analysis and the FoxO3a binding affinity was measured by real-time PCR assay.
HER2 binding affinity was determined in saturation radioligand binding assays using SKBR-3 cells (1.3 × 106 HER2/cell).
After incubation at 37 degrees C for 4 days, the binding affinity was maintained at 60% of initial levels.
The binding affinity was calculated at −8.9 kcal mol−1.
Interestingly, we found that target-binding affinity was modulated by residues in the guide region.
Hydrophobic affinity for these functional groups was anticipated to influence the solubility of graphene in water.
Region-specific binding affinities were analyzed using Student's unpaired t-test.
Linear correlations between the predicted binding scores and the experimentally measured binding affinities were observed.
The process of generating antibodies with increased binding affinities is called affinity maturation.
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