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Based on six different batches of MECA32-Fab-TF, the average tissue factor specific activity was 90 ± 22 μg (mean ± SD) in each mg of MECA32-Fab-TF and the average binding affinity to recombinant PLVAP protein (Kd) was 5.7 ± 1.4 × 10-8 meanean ± SD) using a steady state binding assay and Scatchard analysis (Additional file 2).
Although the obtained ADC lacked a linker, it retained binding affinity to recombinant HER2 (Kd = 0.1 0.5 nmol/L) comparable to that of the parent trastuzumab.
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αHB-EGF LNP-siRNA showed high-binding affinity to recombinant human HB-EGF in a Biacore assay.
Efforts to assess the binding affinity to mouse recombinant CD80 by differential scanning fluorimetry were not successful due to similar melting temperatures T m of the recombinant CD80 and belatacept.
A number of 1,3-dialkyl-9-deazaxanthines (9-dAXs), bearing a variety of N-substituted benzyloxycarbonylamino substituents at position 8, were prepared and evaluated for their binding affinity to the recombinant human adenosine receptors (hARs), chiefly to the hA2B and hA2A AR subtypes.
Their binding affinities to six recombinant human CA isoforms I, II, VI, VII, XII, and XIII were determined by the thermal shift assay (TSA).
The obtained protein exhibits high binding affinity to E2 and the two tags greatly facilitate the application of the recombinant protein.
We measured the binding affinity to HSPG (Sigma-Aldrich, St . Louis MO, USA) and to a c-Met recombinant chimera (R&D Systems), as previously described [ 19].
Recombinant ACBPs, rAtACBP6 as well as rAtACBP4 and rAtACBP5 showed binding affinity to C16:0-CoA, C18 1-CoA, C18 2-CoA and C18 2-CoA.
SS1 and C10 have a high binding affinity to mesothelin (Chowdhury and Pastan, 1999), and SS1 has also been found to be safe in patients when administered as a recombinant immunotoxin (Hassan et al., 2007).
FTC-146, SN-56 and Vesamicol binding affinity to VAChT.
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