In turn, because binding affinities are given in the literature as Pmol, therefore, Pgram can be calculated from the simple equation Pgram = Pmol/MW, which essentially looks the same as the first term in Eq. 3, however, fragmental descriptor refers to a single molecule, while binding affinity refers to a substance.
Total VEGFR1 binding displays a greater range of isoform monotonic behavior than VEGFR2 binding, however, it is limited only up to the VEGF165 isoform, beyond which VEGFR1 binding remains flat or may actually increase with increased matrix-binding affinity (refer to Additional file 1, Figure S3.3Dii).
High affinity refers to binding to receptor-G protein complexes.
Their binding affinity roughly refers to that of full-length probes with more than two mismatches (see Figure 2b and also ref. [13]).
Importantly, some of the available experimental data, such as affinities of binding sites, refer to dynamical processes of gene regulation, which can be thought of as parameters of a dynamical model.
E2 binds directly to ER thereby facilitating ER dimerization and subsequent high affinity binding to ERE (referred to as steroid-dependent ER activation) [13].
To test our hypothesis that class 1 mutations have a more adverse effect on pVHL affinity (referring specifically to the strength of interaction between HIF-2α peptide or protein and pVHL), we conducted both steady-state and kinetic binding experiments.
Independence refers to binding energetics.
The ability of a protein to bind small, drug-like molecules with a high affinity is referred to as "druggability" (ligandability often refers to the more general ability of binding to small molecules).
Biotinylated M1, M2, or M1 + M2 LoNA (mutations on LoNA NCL-binding sites) showed no preferential binding affinity to FBL as compared to WT LoNA (Supplementary Fig. 4c).
As a result, MNPs acquire a dynamic exchange plasma proteins layer, so-called 'corona' (Cedervall et al. 2007), in which competitive displacement of earlier adsorbed proteins by other proteins with stronger binding affinities takes place and is referred to as 'Vroman Effect' (Hirsh et al. 2013).
