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The D12A/D66A mutations cause an increase in binding affinity of similar magnitude for complementary rNTP, relative to the affinity of the wild-type enzyme for rNTP.
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Analogues that retained similar responses as DAA-I in the functional assays exhibited binding affinities of similar magnitude as DAA-I.
Moreover, it is unlikely that attenuation of IKKβ binding affinity of such similar magnitude would occur with two disulfides at such different locations within the NEMO structure.
The protein binding affinity of Sr is similar to that of Ca [56].
For instance, the aforementioned K55 from GNAL can be effectively labeled by GTP and SGTP probes at similar efficiency, suggesting the similar binding affinity of this protein to these two nucleotides.
The binding affinity of raloxifene for ER is similar to that of tamoxifen, and most of the pharmacological data showed similar activity in terms of inhibiting breast cancer cells in vitro and in vivo [ 44].
These in vitro studies found that G5W (isotonic) had equivalent compatibility to sterile water (hypotonic) with similar binding affinity of 90Y to the resin substrate.
These values indicate a similar binding affinity of dATP for the two enzymes, but an ∼100 fold lower catalytic efficiency for the mutant enzyme.
DNA binding affinity of the C141S variant is similar to that of the WT enzyme (28).
In the present study, electrophoretic mobility shift assay (EMSA) showed a similar binding affinity of buffalo HOXC11 protein with the promoter element of LPH gene.
We detect a similar binding affinity of 75 ± 4 nM for unlabeled Brd4(1), 89 ± 5 nM for 3FY-Brd4(1), and 78 ± 2 nM for 5FW-Brd4(1) at 25 °C.
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