Sentence examples for binding affinity of purified from inspiring English sources
Next, we characterized the binding affinity of purified proteins to VSL12 peptide by fluorescence perturbation assays.
The binding affinity of purified monoclonal antibodies was estimated in a competitive ELISA [ 12].
In 0.1% DM, the binding affinity of purified RsTSPO for PK11195 (10 μM) was slightly increased compared to that of 0.2% DM (7 μM), while Fos-Choline-12 lowered the affinity to ∼120 μM.
To directly assess the physical interaction between KpBest and ATP, the in vitro binding affinities of purified KpBest to ATP analogs (ATP, ADP, AMP, and ATPγS) were examined by microscale thermophoresis (MST).
The binding affinity of the lipopeptides for purified LPS was examined using our synthetic dansyl-polymyxin binding assay as previously reported.
The binding affinity of anti-CD20 IgG1 to each purified FcγRIIIa was measured by an FcγRIIIa-binding ELISA assay using plates coated with each shFcγRIIIa-His via anti-tetra-His antibodies (Qiagen) as described previously (Niwa, Hatanaka, et al. 2004).
In vitro binding experiments show that the affinity of purified Hfq truncated at residue 66 (Hfq) for binding cfa is similar to that of the wild-type protein; however, the presence of RydC decreases substantially the binding of Hfq.
However, in our studies with purified RsTSPO, the binding affinity of PK11195 was found to be 10 ± 1 μM, suggesting a binding site in RsTSPO with lower but significant affinity compared to those of the mammalian proteins.
In vitro RNA binding assays show that the RNA affinity of purified RD alone is not as strong as that of the full-length protein.
An in vitro fluorescence binding assay utilizing purified recombinant mitoNEET protein was used to determine the binding affinity of a predicted mitoNEET ligand, and the data obtained is in good agreement with AutoDock Vina results.
