Sentence examples for binding affinity of pol from inspiring English sources
In contrast, the binding affinity of pol β increased 1000-fold withethe 1 nt gap.
However, upon introduction of the 1 nt gap, the DNA binding affinity of pol β increased 200- to 1000-fold.
The reduced binding affinity of pol β to the modified template DNA could be related to the lesion-induced conformational heterogeneity in the active site of the polymerase.
The lesion in the 1 nt gap reduced the binding affinity of pol β by 6-fold for FAAF and 3-fold for FABP, virtually eliminating the nucleotide selectivity of pol β at the lesion site.
The R96G variant had Kd,DNA values 2- to 3-fold higher than wild-type in the presence of either metal, indicating a slight decrease in DNA binding affinity of pol ι by this variant.
The Δ1 25 variation greatly (20- to 29-fold) increased the DNA-binding affinity of pol ι in the presence of Mg2+, which was much less prominent (only about ∼4-fold) in the presence of a low concentration of Mn2+.
Regarding TLS, PCNA mono-ubiquitylation enhances the binding affinity of particular DNA polymerases, called TLS polymerases (Pol- η, Rev1 and Pol- ζ in budding yeast), which are able to substitute the stalled replicative polymerases in a process called "polymerase switch" [ 39].
In conclusion, we have characterized the SPR binding affinity of the mutagenic FABP and FAAF lesions bound to Kf-exo– and pol β.
However, the binding affinity of PDZ domains is known to be regulated through allosteric interactions49.
The binding affinity of CDP2 alone is relatively weak, but it enhances the binding of rITF2 to the TH-dyad.
