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By microcalorimetric and mutational analysis, we determined that one specific hydrogen bond accounts for most of the differences in the binding affinity of individual zinc fingers.
Using the starting pool as control of background, the binding affinity of individual aptamers to U87MG cells and T98G cells was then determined.
We tested the binding affinity of individual aptamers and bidentate aptamer constructs for Ang2 using a fluorescence binding assay.
The convolution filter we employed gives a regional score in such a way that the score is a function of the binding affinity of individual sites, the aggregate number of sites and the spacing of sites within the window.
Our microarray hybridization experiments performed in this study provide quantitative information on the binding affinity of individual mismatched duplexes by means of the hybridization signal intensity (fluorescence of hybridized targets).
However, our results suggest an alternative, wherein the PTM status of Lys (and other residues) may determine the relative binding affinity of individual PABC ligands, allowing regulation of mRNP composition and mRNA fate.
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Nevertheless, it was reported that multivalent binding avidity can be kinetically limited if the binding affinity of an individual receptor-ligand pair is too tight [44, 45].
Well consistent with the above theoretical analysis, our result further suggested that the targeting specificity of the nanoscaled drug delivery systems for a particular cell type can be enhanced by the weaker binding affinity of each individual receptor-ligand pair.
These data and the micromolar binding affinity of the individual peptides suggest that the high-affinity and high specificity of the synbody is the product of a bivalent interaction.
(F ) The binding affinity of Bub1 Bub3 for individual MELTP is high.
The binding affinity of Bub1 Bub3 for individual MELTP repeats of Spc105/Knl1 is high, matching the highest binding affinities measured for SH2-phosphopeptide interactions (Seet et al., 2006).
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