Collectively, these data suggest that the involvement of acidic residues E57 and E58 in the QacR multidrug binding pocket is not absolutely required for the neutralization or binding affinity of either cationic drug Be or Dq.
Furthermore, it has been shown that the presence of 8-oxodG as a templating base does not reduce the binding affinity of either yeast Polη or the model Y-family polymerase Dpo4 for a DNA substrate, suggesting that the Y-family polymerases are well suited to accommodate this small lesion within their relatively large active sites, without a reduction in DNA binding capacity.
As for the soluble species, total free VEGF in all compartments decreased with increasing VEGF-binding affinity of either VEGFR1 or VEGFR2 – presumably through enhanced internalization of complexed VEGF (Fig. 6B).
Briefly, the differences in molarity of sucrose and sucralose reflect differences in ligand-binding affinity of either sweetener to taste receptors, and were chosen among the plateau values of behavioral dose-response curves (preference for either sweetener versus water in Domingos et al., 2011).
At the 20 100 mM range, salt does not seem to affect the ssDNA-binding affinity of either the wild-type or variant forms of HsmtSSB.
The results showed no difference in binding affinity of rTFs with either VP16 or VP64 (Figure 2), suggesting that the ADs in such constructs performed equally well, at least with respect to DNA-binding ability.
In practice, small ligand molecules may be employed to regulate and tune the binding affinity of regulatory proteins, being either monomers or dimers.
Our findings are also in agreement with a study examining binding affinity of several phytoestrogens to either ERα or ERβ; compared to the E2 standard (set as 100%), relative binding affinity of genistein, biochanin A, coumestrol, and diadzein were greater to ERβ than to ERα [ 64].
The p38 MAPK controls the binding affinity of ARE-binding proteins either directly or through activation of downstream kinases (e.g. MK2, MK3 and the MAPK-interacting kinases) [25], [26].
It has been hypothesised that the rs266882 polymorphism may be associated with serum levels of PSA through higher binding affinity of the ARE1 with either the A or G allele.
K250 and these degrons are conserved in vertebrates, suggesting that BubR1 acetylation might regulate SAC activity by controlling either the binding affinity of BubR1 to the Cdc20 coactivator (Burton and Solomon, 2007) or the APC/C-dependent destruction of BubR1 (King et al, 2007).
