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The binding affinity of dye molecules is indicated by high k L values.
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Fig. 3A illustrates the binding affinity of each dye for the IQ peptide on phage, with GH680 exhibiting the highest affinity.
Another possible contributing factor to PCR inhibition is the high binding affinity of the dye to dsDNA, particularly to short dsDNA fragments.
Because the duplexes formed from mis-priming are very short fragments, the binding affinity of a qPCR dye to short dsDNA fragments should correlate well with the dye's tendency to promote nonspecific amplifications.
However, the binding affinity of PDZ domains is known to be regulated through allosteric interactions49.
This binding is explained by the broad binding affinity of lectin.
The binding affinity of 4-[18F]FEBZA wassessedsed using a homologous competitive binding assay.
Due to different binding mechanisms, the binding affinity of various TSPO radioligands may vary significantly.
Competitive ligand-binding assays measure the binding affinity of a substance to an (isolated) receptor.
The binding affinity of CDP2 alone is relatively weak, but it enhances the binding of rITF2 to the TH-dyad.
Mal III has the highest binding affinity, whereas the binding affinity of Mal II and Mal IV are nearly identical and the binding affinity declines from Mal V to Mal VII.
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