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A fluorimetric ECM cell adhesion array kit (Chemicon International) allowed us to screen for and quantitate the binding affinity of cells to 7 different human ECM proteins.
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RGD peptide treatment significantly abrogated cell-ECM binding in all ADAMTS-12 and LacZ transfected cell populations, whereas ECM binding affinities of cells cultured in untreated media or with the control peptide were not affected (Figure 5B).
Since CD28 ligation enhances the binding affinity of T cells to endothelial cells [ 34], the trafficking of T cells lacking CD28 between blood and tissues may be altered.
The EGF receptor numbers and binding affinity of these cells were the same as for the parental cells.
The binding affinity of the cells was increased by treating the cellulose with phosphoric acid.
When factors such as CD8 and TCR expression levels are considered, the relative median fluorescence intensity (MFI) of multimerized peptide-MHC (tetramer) staining reflects the binding affinity of T cells [ 26, 32– 32].
Many other potential impacting factors for immunogenicity, e.g., initial number of naive T and B cells and number and binding affinity of B-cell epitopes, are designed as integral parts of the model structure; these can also be conceivably informed by conducting appropriate experiments.
This property would help to explain the apparent binding affinity of EPEC for cells that are 'less differentiated' in vivo i.e. adenocarcinoma, fetal and possibly proliferative crypt cells.
In contrast, the hCG binding affinity of the granulosa cell was equivalent at both stages of follicle development and remained unchanged after 6 days of culture (mean apparent Kd = 2.9 x 10-10M; n=27.
The binding affinity of a series of cell-penetrating peptides (CPP) was modeled through docking and making use of the number of intermolecular hydrogen bonds, lipophilic contacts, and the number of sp3 molecular orbital hybridization carbons.
To determine the binding affinity of the aptamers, positive cells were cultured overnight and the cells dissociated using non-enzymatic dissociation buffer and/or short time (30 sec) trypsin treatment.
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