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No significant effect of peptides on anti-CCR5 binding was observed indicating that the peptides bind to an epitope distinct from the antibody-binding site or their binding affinity is not sufficient for effective competition with this particular antibody.
However, it has become known that a sufficient DNA binding affinity is not always obtained.
On the other hand, the antiviral activity of interferons on WISH cells seems to change in accordance to the binding affinity towards IFNAR1 only as long as the binding affinity is not beyond twofold of the wild-type.
The binding affinity of JEV SA14 and SA14-14-2 to thostost cells is similar (Fig. 2B), so the binding affinity is not the main cause of JEV SA14-14-2 neurovirulence attenuation.
Fortunately, the calculation of ligand binding affinity is not necessory for the objective in this investigation that is to explore the possible dissociation pathways of typical type II inhibitor imatinib from its targeting protein kinases c-Kit and Abl.
For EpanSet4, binding affinity is not available, and we used the binary labels in the dataset directly.
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Moreover, the binding responses were similar to values obtained for the interacting proteins, indicating that the binding affinity was not affected by the fusion.
Immuno-EM confirmed that recombinant ACE2 protein bound to virions, and that binding affinity was not affected by γ-irradiation (Figure 1B).
Thus, the binding affinity was not significantly affected by peptide length as long as the peptide contains the nine amino acids in the DxETGE motif.
Although the binding affinity was not altered by this H4 substitution in vitro, Asf1 sequesters H4G94P to a greater degree than WT H4 in vivo.
These measurements indicated high-affinity (KD = 0.7 nM) recognition of the CCAAT box by the A. fumigatus CBC independent of the presence of the 3′-submotif, i.e. its binding affinity was not affected by truncation of the 3′-submotif (compare first column in Fig 6B).
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