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MOP replaced NPN at pH 7 (Fig. 5A), but not at pH 5 (Fig. 5B) thus confirming that binding affinity is lost or is very weak at low pH.
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The glycan GlcNAc-(LacNAc 3 represented the highest binding affinity among the 465 candidates; however, the high binding affinity would be lost if the terminal GlcNAc was removed.
The apparent binding affinity was derived from 3 or more experiments.
(h) Granulosa cells were harvested for ChIP analysis and the FoxO3a binding affinity was measured by real-time PCR assay.
HER2 binding affinity was determined in saturation radioligand binding assays using SKBR-3 cells (1.3 × 106 HER2/cell).
After incubation at 37 degrees C for 4 days, the binding affinity was maintained at 60% of initial levels.
This complex dissociates when the antibody is affinity-purified, so binding is lost.
Presumably, the integrity of the substrate binding site is lost when these residues are mutated leading to lower affinity for substrate.
As the low‐affinity binding site is lost in gephyrin‐G375D, we conclude that Gly375 is likely an integral part of this binding site.
Also CAP binding is lost.
The process of generating antibodies with increased binding affinities is called affinity maturation.
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