Sentence examples for binding affinity for ssDNA from inspiring English sources
Several mutant enzymes had altered binding affinity for ssDNA.
Rad52 has greater binding affinity for ssDNA than dsDNA (46, 47) and appears to have two separate binding sites for each in the N-terminus of Rad52 (48).
This analysis showed that peptide 5015 containing the RRR and NLS stretches, and peptide 10987 containing the downstream-finger, have, among the assayed peptides, the highest binding affinity for ssDNA (Kd=0.21±0.06 μM and Kd=0.30±0.02 μM respectively).
In the accompanying manuscript, Kuper et al suggest that F133 whose mutation displays decreased binding affinity for ssDNA without affecting unwinding activity, contributes to the wedge (Kuper et al), and therefore might be functionally redundant with F131.
These data support the notion that the RRM domain of HvGR-RBP1 exhibits micromolar binding affinity for 6nt ssDNA targets in these in vitro experiments.
Such a "scrambled" 6-nt oligonucleotide ssDNA sequence resulted in significantly weaker binding to N- HvGR-RBP1 (∼550 μM Kd compared to equilibrium dissociation constants of ∼4 43 μM for other ssDNA ligands), indicating that the nucleotides adjacent to the CTG element are indeed important modulators of the RRM binding affinity for its ssDNA target.
Figure 4(b) shows that RtelN could also bind dsDNA with the similar binding affinity as for ssDNA.
Interestingly, the ssDNA-binding protein hSSB1, which is highly conserved in Xenopus, has a higher binding affinity for poly-pyrimidine than for poly-purine containing ssDNA molecules (Richard et al, 2008).
Surprisingly, in our hands the S369P variant had a clearly increased affinity to ssDNA in contrast to the results of Korhonen et al. (29), who reported a decreased binding affinity for the same variant.
To further verify that the DDX3ΔINS had a reduced binding affinity for nucleic acids also in the absence of catalysis, an electrophoretic mobility shift assay was performed, in the presence of various amounts of DDX3wt or DDX3ΔINS and a fixed dose of a 5'-labelled ssRNA or ssDNA oligonucleotides.
