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The tandem motifs show higher binding affinity for mono- or polyUb than any individual UIMs.
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The GlcNAc-binding specificity of BLL was determined by frontal affinity chromatography, and BLL was shown to have high binding affinity for mono-, bi- and tri-antennary agalactosylated glycans [ 31], whereas AAL-2 showed high binding affinity for mono-terminal non-reducing GlcNAc residues.
17f One report even describes a step-by-step decrease in binding affinity from mono- to di- to tetra-valent compounds.
This compound had a higher apparent binding affinity for binding His tags (0.1 0.5 µM in this study and [12]) compared to mono-NTA fluorophore conjugates which have apparent Kd values between 1 4 µM [13], [18].
We then used the reconstructed LBD structure to design new mono-, bi- and tricyclic amine-bearing compounds that might be expected to show greater binding affinity for the LBD.
c Targets detected with no significant binding affinity for CIS22a.
Moreover, the phosphorylation modification of FUNDC1 affects its binding affinity for LC3B and regulates selective mitophagy.
Bis-DOTA-hypericin had a selective binding affinity for PS and PE phospholipids [45].
The tetradecapeptide bombesin (BN) shows high binding affinity for these BN2/GRP receptors.
These VHHs were selected based on their specificity and binding affinity for their targets.
Our molecular docking platform explored the binding association of various drugs inside the binding pocket, but did not analyze the peptide's binding affinity for these drugs.
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