For SLII, the difference in binding affinity with or without U2A′ is small, such that U2A′ binding affinity could not be extracted from these experiments.
In this way, binding affinities could be estimated for compounds as weak as 30 mM.
A similar mechanism may occur at rs143383, with methylation increasing the repressive activity of SP3 despite reducing SP3 binding affinity, and this could explain why SP3 represses the methylated 5′UTR luciferase plasmid more strongly than the unmethylated plasmid.
GABAAR scaffold-binding affinity could be regulated by PKC.
In both cases, burial of the Cl atom within the binding site could explain the observed sensitivity of the binding affinity to Cl deletion (Table 2).
It is possible that the higher affinity provided by arginine residues in a HS-binding interface could explain why FGF18 has a lower stringency for HS sulfation than other FGFs.
The higher binding affinity may explain why thallium could not replace sodium in the heavy-atom soaking experiment in GltPh (ref. 9).
This slightly lower labeling efficiency for Cy3-hLXRα could yield an underestimation of the binding affinity and explain in part some of the differences noted between the two assays.
These findings along with aflibercept's higher binding affinity help explain the observed differences in plasma VEGF levels.
The high affinity of mAb TA12 could explain the strong neutralising effect observed in vivo.
Such an oligomer complex could explain why in vitro receptor binding affinities (∼1 µM) do not correlate well with the measured responses of plants to chitin elicitation at nM concentrations (Zhang et al., 2002).
