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General fitting and steady-state binding affinity calculated with Biacore evaluation Version 3.0 software helped release a relative high binding affinity for tubulin with the dissociation constant (Kd) value of 11.1 µM, which was two-fold lower than that of colchicine (24.6 µM).
Similar(59)
The binding affinities calculated based on the free-energy profiles are found to be in excellent agreement with experimental measurements for different-sized NCs.
These values are similar to binding affinities calculated for human serum retinol binding protein (hRBP) (Cogan et al., 1976).
From the second binding event a binding affinity has been calculated, with a Kd of 0.6 µM.
Based on the docking model of AtLYK5 with chitooctaose, the binding affinity was calculated at −8.9 kcal mol−1, a value comparable to the computational binding affinity of ECP6 (−9.0 kcal mol−1).
The binding affinity was calculated as −9.59 kcal/mol by AutoDock; the Ki value was 94.12 nanomolar.
The binding affinity was calculated at −8.9 kcal mol−1.
(B ) The binding affinity was calculated at −8.9 kcal mol−1.
The approximate binding affinity was calculated as in eq 7 (see Methods).
SPR and solid-phase binding assays show that Cyr61 interacts with immobilized SMTB 1-44 domain [15] [20], with a similar affinity calculated for VTNC.
Peptides were incubated with LatB-bound G-actin (peptide 0.5 μM; LatB actin 0 59 μM) and the actin-binding affinity was calculated from anisotropy by nonlinear regression.
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