Sentence examples for binding affinity and sequence from inspiring English sources

The key characteristics of each parent protein (catalytic efficiency of barnase, DNA binding affinity and sequence specificity of GCN4) are retained in the chimera.

Two non-self-complementary 17-mer double-stranded DNA (dsDNA) with four different central base pairs were designed to systematically investigate the binding affinity and sequence specificity of berberine with dsDNA by capillary zone electrophoresis (CZE).

Based on in vitro biological activity, antigen binding affinity, and sequence analysis, we selected EBV321 for humanization.

The differences in binding affinity and sequence selectivity for these two compounds, which differ only at the amidine group, are very striking.

Moving one terminal amidine to a meta position (DB2289) results in a dramatic decrease in binding affinity and sequence selectivity, and this indicates that not only the shape of the main scaffold but also the position, molecular curvature, and the distances between two positively charged amidine groups play crucial roles in ligand DNA interaction.

Their DNA-binding affinities and sequence specificities were evaluated by SPR and Bind-n-Seq analyses.

It has enhanced binding affinity, and it showed comparable sequence specificity to its monoamino/monocationic counterpart f-IPI 1. Results from CD and DNase I footprinting studies confirmed the minor groove binding and selectivity of polyamides 1 and 2 for the cognate sequence 5′-ACGCGT-3′.

Consistent with the WebLogo analysis of the consensus sequence and analyses by an EMSA and ITC, mismatches at the edges of the motifs had a weaker impact than those in the middle of the motif on the binding affinity, and a GC-rich sequence in the spacer or immediately following the second motif significantly reduced the binding affinity.

Changes to another key region of the hairpin controlling ribonuclease binding affinity and containing the aptamer sequence resulted in more notable horizontal scaling, as was expected; in addition, changes in vertical extension and leakage levels were again observed, likely resulting from nucleotide modifications to the hairpin stem that were required for variable aptamer integration.

Comparisons between TRF1 and TRF2 must be made with caution, as despite the comparable DNA binding affinities and telomeric sequence specificities (Table 1) their binding behavior is otherwise grossly different upon examination via an EMSA.

Moreover, the link between sequence, binding affinity, and fitness is experimentally accessible.