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Open image in new window Figure 2 Neurovirulence, binding affinity and fusion activity of JEV SA14, SA14-14-2 and the revertant viruses.
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The binding affinity and specificity of rGel-based fusion toxins containing the combinations of CPE290-319, E9 and R9 were evaluated by ELISA on both CLDN3 and CLDN4-positive 2008 cells and CLDN3-knockdown cells.
We have engineered the Shh-Fc fusion protein in order to improve binding affinity and increase systemic exposure in animals.
Our results show that fusion order is important for MBP binding affinity and HepA activity, while the linker sequences at domain junctions have significant effects on protein expression level, HepA activity and thermostability as well as GFP fluorescence.
In vitro fluorescence assays demonstrated that the binding of these dyes to the FAP affibody fusions produced thousand-fold fluorescence enhancements, with high binding affinity and fast association rates.
The CtxB-VP1 fusion protein produced in the chloroplast retained both GM1-ganglioside binding affinity and antigenicity.
The silkworm bioreactor produced this fusion protein vaccine as the pentameric CTB insulin form, which retained the GM1-ganglioside binding affinity and the native antigenicity of CTB and insulin.
A fusion which incorporated the C-terminus addition of a positively charged tail (B5-GS3K) improved biosensor sensitivity to CEA while also retaining the sub-nanomolar binding affinity and thermal stability of the unmodified sdAb.
Computational models rationalize the binding affinity and the inhibition mechanism.
The binding affinity and molecular conformations are discussed.
Their multiligand chelation ability enhances their binding affinity and stability.
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