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Furthermore, for each mutation, changes in binding affinities were mirrored by equivalent changes in ligand sensitivity.
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Region-specific binding affinities were analyzed using Student's unpaired t-test.
Linear correlations between the predicted binding scores and the experimentally measured binding affinities were observed.
The process of generating antibodies with increased binding affinities is called affinity maturation.
Thus native binding affinities are measured.
BtR175-binding affinities were assessed by Biacore.
The apparent binding affinity was derived from 3 or more experiments.
(h) Granulosa cells were harvested for ChIP analysis and the FoxO3a binding affinity was measured by real-time PCR assay.
Importantly, while target antigen affinity was completely impaired, CD3 binding affinity was conserved in each molecule.
HER2 binding affinity was determined in saturation radioligand binding assays using SKBR-3 cells (1.3 × 106 HER2/cell).
After incubation at 37 degrees C for 4 days, the binding affinity was maintained at 60% of initial levels.
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