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High binding affinities were confirmed for all 30 mutants containing loop sequences that differed from those of wild-type.
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The direct binding affinity was confirmed by surface plasmon resonance (SPR).
The determined binding affinity values were confirmed using a fluorescent competitive binding assay and isothermal titration microcalorimetry.
Linear correlations between the predicted binding scores and the experimentally measured binding affinities were observed.
Furthermore, the results of inverse docking (INVDOCK) analysis suggested that WB could bind to Ras GTP, and the direct binding affinity was also confirmed by surface plasmon resonance (SPR).
The gel filtration data therefore indicate that the mutations reduce binding affinities, which was confirmed in the case of Smc1 M665R by a pull-down assay.
The process of generating antibodies with increased binding affinities is called affinity maturation.
Thus native binding affinities are measured.
The fitted binding affinities are shown within the corresponding figures.
Hydroxyapatite (HA) coatings composed with bisphosphonates (BPs) which have high mineral-binding affinities have been confirmed to successfully enhance implant stability.
As expected, AM580 alone enhances the heterodimer affinity for the SRC-1 NR2 peptide (kd = 0.19±0.02 µM), while in conjunction with LG100754 similar results in terms of stoichiometry and binding affinity were seen (kd = 0.17±0.01 µM) (Figure 7), confirming that AM580 promotes the binding of the SRC-1 NR2 peptide to the RARα subunit.
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