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Two anti-TNT antibodies with differing binding affinities were compared in the reversed-displacement assay format, and a correlation between affinity and detection limits was observed.
Binding affinities were compared with one-way ANOVA Tukey's post-hoc tests.
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Most of these interactions are probably shared by both muscle actinin isoforms, though this has often not been explicitly tested, nor have actual binding affinities been compared.
Region-specific binding affinities were analyzed using Student's unpaired t-test.
Linear correlations between the predicted binding scores and the experimentally measured binding affinities were observed.
The binding affinities can be compared to the affinity constants determined for MBP from the association and dissociation rates (kon/ koff) and measured with stopped-flow fluorescence spectroscopy.
Moreover, IL-1F10, in addition to IL-1α, IL-1β and IL-1Ra, also can bind to IL-1R1, although its binding affinity is low compared with IL-1β and IL-1Ra [64].
When two mutations at different loops were integrated into one molecule, no reduction in binding affinity was observed compared with wild-type sequences.
In case of RGPD/HMPR double mutant, an increase in binding affinity was observed compared with the original mutants (Table 2).
While SR RS2) is a substrate for both kinases, the binding affinity is reduced compared with the wild-type kinase since we were unable to saturate the kinases in plots of initial velocity versus substrate.
Due to high surface curvature of NP as compared to the bulk materials, their protein binding affinities are distinct from bulk materials of the same composition [56].
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