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A solution- and solid-phase method is described and binding affinities of representative compounds are presented.
A solution- and solid-phase procedure is described and binding affinities of representative compounds presented.
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In addition to measuring the functional activity of these compounds at the CB1R and CB2R, the binding affinity of representative examples at both receptors was determined, in order to measure Ki values.
A systematic analysis of PhoP binding affinity of a representative subset of potential binding sites by ITC or an EMSA combined with a bioinformatic approach is necessary to establish a set of rules to identify true PhoP-binding sites from the results of a whole genome promoter search.
The dose-dependant glycan binding data of the described HAs were used to calculate Kd' and n values (n ∼1.3 for all the HAs) by fitting the binding data to the Hill equation (for multivalent binding) and this was then used to generate theoretical binding curves to clearly distinguish the relative binding affinities of WT and mutant H2 HAs to representative avian and human receptors (Figure 6).
(D) ITC measurements of binding affinities of MRG1CD to tri-methylated histone peptides.
The most notable changes were the improved binding affinities of LvIA (N9A) and LvIA (D11A).
The binding affinities of EBOV GP to these chimeras were determined by SPR.
The binding affinities of the designed tripeptides are all superior to the binding affinities of their known tripeptide ligands.
However, binding affinities of the agents are different.
The binding affinity of the most representative molecules (8, 9, 19, 20, 21, 23 and 25), including analogues 8 and 21 without the side chain, for the estrogen receptor α (ER)† was determined.
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