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Comparing the types of ligands reveals that the binding affinities of each peptide for the two adsorbents are similar.
The overall peptide binding preferences for the DR1201 allele were deduced by incubating these peptides in competition with the reference H1MP40 54 to determine the relative binding affinities of each to recombinant DR1201 protein.
We then measured the binding affinities of each construct using a bead-based fluorescence assay.
If we were to investigate two or more transcription factor binding motifs using this approach, it would be necessary to know more about the relative binding affinities of each, or to assume equal affinity, for better or worse.
13 Binding affinities of each individual bisphosphonate also contribute to their antiresorptive potency.
Similar(55)
The binding affinity of each nanoconjugates for TNT detection by using Langmuir adsorption methods has also been investigated.
Then, a single-point Kd calculation method (Qin et al. 2015) was employed to estimate binding affinity of each ligand to the receptor (Table 1).
Well consistent with the above theoretical analysis, our result further suggested that the targeting specificity of the nanoscaled drug delivery systems for a particular cell type can be enhanced by the weaker binding affinity of each individual receptor-ligand pair.
Fig. 3A illustrates the binding affinity of each dye for the IQ peptide on phage, with GH680 exhibiting the highest affinity.
To determine the binding affinity of each selected or recombinant clone, the apparent Kd of the individual clones were assessed by phage ELISA.
Under this assumption, the peptide binding affinity of each SH3 domain can be expressed as a 20×10 matrix where each element represents the binding energy of a particular amino acid at a specific position of the peptide.
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