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Methods for measuring in vitro TF-DNA binding affinities have also been developed [9] [11].
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Several mutants of TBP with altered DNA binding specificity and affinity have also been reported [ 26].
The binding affinity data have also been derived from the PDB and are included as one of the browse capabilities.
Using small peptidomimetics, correlations between higher-order multivalency, receptor binding affinity, and tumor uptake have also been uncovered [44], [45].
Recently, differences in the binding affinities of mammalian H1 variants have also been shown both in vitro [ 30] and in vivo [ 31].
Receptor-specific fluorescent ligands with high binding affinity and low off-rates have also been found to be suited for receptor pulse labeling [ 100].
However, cases in which RBPs have high binding affinity to RNAs forming hairpin loops have also been reported [ 7].
In addition, a low-affinity RACK1-binding site has also been located in the PDE4D5 catalytic domain [ 11].
Methotrexate-alpha-arginine and fluoresceinated have also exhibited good binding affinity − 38.85 and − 37.27 respectively.
Both studies have also looked at the binding affinity between Bax and the Bim BH3 peptide biophysically, and only low (μM) affinities were measured.
The use of AChBPs structure as a surrogate to predict binding affinity to α7 nAChR has also been investigated.
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