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ITC measurements for the binding affinities between the specific RNA and the protein were performed at 30°C and 10°C using Auto-iTC100 titration calorimetry (MicroCal/Marvin).
The binding affinities between the inhibitor and Aβ as well as the inhibitor to itself have been estimated using Umbrella Sampling calculations.
To confirm the residues of LC3B that interact with FUNDC1 Ser13, we introduced alanine mutations into region 1 and region 2 of LC3B and performed ITC assays to determine the binding affinities between the unphosphorylated FUNDC1 peptide and LC3B mutants.
Using this receptor-focused approach, it is possible to capture the observed fold change in binding affinities between the wild-type and disease-centric mutations in ABL kinase for Imatinib and the second-generation ABL drugs.
This should allow us to rank the binding affinities between the different coiled-coil structures by their binding free energies.
To analyze the binding affinities between the aptamers and the E1E2 glycoproteins of different genotypes (genotypes 1 through 6), 96-well ELISA plates were pretreated by coating them with 100 µg/mL poly-L-lysine.
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These GBP-fusion proteins can directly bind onto the gold surface via the high binding affinity between the GBP and the gold surface, while at the same time, orient the recognition sites toward the sample for target binding.
Spectroscopic characterization indicated that MPHs are well distributed, with a strong binding affinity between the GO nanosheets.
The degradation rate constants of the PCBs decreased as binding free energy increased (i.e., the binding affinity between the ligand and receptor decreased) [19].
This confirms that the dimensionless quantities RM and RN are useful parameters that allow for quantifying the binding affinity between the chemically modified surface and AuNPs.
This is explained by reducing the binding affinity between the siRNA strands to the complex, thereby enabling siRNA release after cellular internalization.
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