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The latter hypothesis has been argued previously in the report of altered affinity for H]DAMGO in tissue from people who died as a result of suicide [ 14] as changes in binding affinities are usually due to competition from endogenous ligands [ 40, 41].
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Thus native binding affinities are measured.
The fitted binding affinities are shown within the corresponding figures.
Linear correlations between the predicted binding scores and the experimentally measured binding affinities were observed.
The binding affinities were evaluated over a range of 2.5 40 mmol/L concentrations.
The binding affinities were estimated as a Docking score (Glide score + Epik state penalties).
The residual binding affinities were computed as: Delta G^{res} = Delta G^{exp } - Delta G^{pred} (5).
The process of generating antibodies with increased binding affinities is called affinity maturation.
HER2 binding affinity was determined in saturation radioligand binding assays using SKBR-3 cells (1.3 × 106 HER2/cell).
This indicates that even though the binding location of DB00631 fluctuates with different peptides, the overall binding affinity is conserved.
Importantly, while target antigen affinity was completely impaired, CD3 binding affinity was conserved in each molecule.
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