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Three separate point mutations causing three independent amino acid changes (nonsynonomous mutations) accumulated in the same sequence and enriched throughout the selection protocol, suggesting that these changes confer binding advantages.
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However, the latter one does not appear at the PA-CMG2 interface (Figure 5B), thus giving TEM8 some extra binding advantage over CMG2.
To confirm in vivo target specificity of EC0746, we conducted competition studies in AIA rats using a benign folate-containing competitor (EC0923) to block the FR binding advantage of EC0746.
Tijink, B. M. et al. Improved tumor targeting of anti-epidermal growth factor receptor Nanobodies through albumin binding: taking advantage of modular Nanobody technology.
This demonstrates that PIII treatment of polystyrene improves the polymer's tropoelastin binding properties, with advantages for tissue engineering and prosthetic design.
The success in the identification of a new P2Y14R fluorescent agonist with low nonspecific binding illustrates the advantages of rational design based on recently determined GPCR X-ray structures.
The immunoassay methods based on antigen antibody binding have the advantages of simple, rapid and easy to operation, and appear an useful tool for on-site detection of OTA [8 11].
Because the CRISPR system only requires a short sequence of RNA to target nuclease binding, it provides advantages over established genome-editing systems like TALENs and zinc-finger nucleases (ZFNs) which require unique protein domains to achieve binding to the desired sequences.
Tubulin inhibitors that bind to the colchicine binding site have therapeutic advantages over taxanes and vinca alkaloids, for example, they can be administered orally, have less drug-drug interaction potential, and are less prone to develop multi-drug resistance.
Further analysis revealed that ganoderic acid DM is a potential source of anti-influenza ingredient, with novel binding pattern and advantage over oseltamivir.
These systems use optical signaling methods to reveal the binding event, taking advantage of molecular biological techniques to optimize the signal.
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